“Recent Developments in AP/MALDI MS Imaging: Spatial Biomolecule Analysis Innovations for studying Biology/Medicine”
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1638 NW 10 Avenue, Miami, FL 33136
http://Join Zoom Meeting https://miami.zoom.us/j/98840380815 Meeting ID: 988 4038 0815For over 40 years, matrix-assisted laser desorption/ionization (MALDI) has revolutionized mass spectrometry of biological tissues. Recently, advancements in atmospheric pressure MALDI sources have made this technology compatible with high-resolution instruments like orbitraps. While formaldehyde fixation and paraffin embedding (FFPE) are preferred for light microscopy, FFPE tissue processing was not initially ideal for mass spectrometry imaging (MSI). However, with modern mass spectrometers and refined sample processing techniques, a new approach called mass spectrometry histochemistry (MSHC) has emerged. By revisiting FFPE samples stored in tissue banks worldwide, researchers can now successfully image biologically relevant molecules in histological sections without the need for enzymatic assistance. Recent experiments in MSHC have demonstrated the direct imaging of endogenous peptides, metabolites, and even neurotransmitters in FFPE sections. These advancements have been made possible by combining AP/MALDI with various orbitrap HRMS systems, showcasing enhanced sensitivity, accuracy, and data acquisition speed with each new instrument generation. MSHC enables innovative in situ biomarker research in pathology by providing cellular spatial resolution for top-down peptide and metabolite analyses in well-documented human clinical samples.
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