Microscopy-Guided Subcellular Spatial Proteomics Discovery: The Syncell Microscoop Platform
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1430 NW 11th Avenue, Miami 33136
https://miami.zoom.us/webinar/register/WN_X8lABPWvT-ykgGoUjuHQtgTraditional proteomics approaches often rely on predefined panels or antibody-based enrichment, which introduces bias since only known or expected proteins are detected. The Syncell Microscoop platform labels all proteins without pre-selection in specific regions of interest; these proteins are then subjected to mass spectrometry analysis for identification and characterization. The Microscoop allows mass spectrometry to capture low-abundance or rare proteoforms and reveal previously unknown protein constituents with higher dynamic range and specificity than laser microdissection, antibody-based imaging workflows, or proximity labeling methods. This seminar will provide an overview of how the Microscoop provides spatial protein purification through microscopy-guided in situ subcellular photo-biotinylation of proteins at user-defined regions of interest, one field of view at a time across thousands of fields of view, with FFPE or fresh frozen tissue samples and fixed cell culture samples. Together with mass spectrometry analysis, the Microscoop enables the discovery of subcellular proteomes with high sensitivity, specificity, and resolution, facilitates the elucidation of new cell types and states by identifying unique protein markers, supports the discovery of novel biomarkers and therapeutic targets, and helps to uncover the players of the cancer microenvironment.
This Sylvester Shared Resources Advanced Biomedical Technology Seminar is jointly hosted by the Sylvester Flow Cytometry Shared Resource (FCSR) and the developing Sylvester Cancer Proteomics Shared Resource (CPSR).
Speaker:
Azad Gucwa, Ph.D.
Senior Field Application Scientist
Syncell Inc.
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