McKnight Vision Research Seminar- Presenter: Dr. Judith West-Mays, Professor, Department of Pathology and Molecular Medicine, McMaster University
Wednesday, March 22, 2023 12pm to 1pm
About this Event
“Genes involved in Anterior Segment Dysgenesis and Glaucoma”
Our recent work has shown that expression of Activating protein-2 beta (AP-2b) transcription factor in the developing periocular mesenchyme (POM) is critical for proper anterior angle formation in that its targeted disruption in mice results in a closed angle phenotype and glaucoma. To that end, we believe that the conditional knockout mouse strains we have generated are currently among the best models for studying developmental glaucoma. I will describe and compare the two conditional mutants we have created and outline the advantages of each in studying anterior segment dysgenesis and glaucoma. Our current studies are further aimed at determining how the patterns of gene expression in the anterior angle tissues are disrupted by the loss of AP-2b. To carry this out we have performed scRNAseq analyses on eye tissues from our models. The candidate genes revealed are currently being followed up using RNAscope probes and RT-PCR analyses. The results will be discussed in the context of known regulators of anterior segment development that are expressed in the POM to construct a gene regulatory network that can be applied to the analysis of glaucoma.
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https://miami.zoom.us/j/94036094258
Meeting ID: 940 3609 4258
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